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UniProt functional annotation for Q13564 | ||
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| UniProt code: Q13564. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. {ECO:0000269|PubMed:10207026, ECO:0000269|PubMed:10722740, ECO:0000269|PubMed:12740388}. |
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| Activity regulation: | |
Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity. |
| Pathway: | |
Protein modification; protein neddylation. |
| Subunit: | |
Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2. {ECO:0000269|PubMed:12646924, ECO:0000269|PubMed:14690597, ECO:0000269|PubMed:15361859}. |
| Subcellular location: | |
Cell membrane {ECO:0000269|PubMed:14557245}. Note=Colocalizes with APP in lipid rafts. |
| Tissue specificity: | |
Ubiquitous in fetal tissues. Expressed throughout the adult brain. {ECO:0000269|PubMed:8626687}. |
| Ptm: | |
Ubiquitinated by TRIP12, leading to its degradation by the proteasome. {ECO:0000269|PubMed:18627766}. |
| Miscellaneous: | |
NAE1 and UBA3 correspond to the N-terminal and the C- terminal part of yeast UBA3. In yeast the two subunits form a single polypeptide chain. |
| Similarity: | |
Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.