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PDBsum entry 3czv

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Lyase PDB id
3czv
Jmol
Contents
Protein chain
258 a.a.
Ligands
AZM ×2
GOL
Metals
_ZN ×2
Waters ×457

References listed in PDB file
Key reference
Title Crystal structure of human carbonic anhydrase xiii and its complex with the inhibitor acetazolamide.
Authors A.Di fiore, S.M.Monti, M.Hilvo, S.Parkkila, V.Romano, A.Scaloni, C.Pedone, A.Scozzafava, C.T.Supuran, G.De simone.
Ref. Proteins, 2008, 74, 164-175. [DOI no: 10.1002/prot.22144]
PubMed id 18618712
Abstract
The cytosolic isoform XIII is a recently discovered member of the human carbonic anhydrase (hCA, EC 4.2.1.1) family. It is selectively expressed among other tissues in the reproductive organs, where it may control pH and ion balance regulation, ensuring thus proper fertilization conditions. The authors report here the X-ray crystallographic structure of this isozyme in the unbound state and in complex with a classical sulfonamide inhibitor, namely acetazolamide. A detailed comparison of the obtained structural data with those already reported for other CA isozymes provides novel insights into the catalytic properties of the members of this protein family. On the basis of the inhibitory properties of acetazolamide against various cytosolic/transmembrane isoforms and the structural differences detected within the active site of the various CA isoforms, further prospects for the design of isozyme-specific CA inhibitors are here proposed. Proteins 2009. (c) 2008 Wiley-Liss, Inc.
Figure 3.
Figure 3. Superposition of the hCA XIII (red) and hCA II (green) C trace. The zinc ion and its three histidine ligands are represented in ball and stick.
Figure 6.
Figure 6. Schematic view of the hCA II (A) (PDB code 1CA2),[27] hCA I (B) (PDB code 2CAB),[28] hCA XIII (C), and hCA III (D) (PDB code 1Z93)[26] structures. The zinc ion and its three histidine ligands (H94, H96, and H119) are shown. For each isozyme, the histidine residues participating to the proton-transfer reaction are also reported by PyMOL sphere representation (http://pymol.sourceforge.net/).
The above figures are reprinted by permission from John Wiley & Sons, Inc.: Proteins (2008, 74, 164-175) copyright 2008.
Secondary reference #1
Title Refined structure of human carbonic anhydrase ii at 2.0 a resolution.
Authors A.E.Eriksson, T.A.Jones, A.Liljas.
Ref. Proteins, 1988, 4, 274-282.
PubMed id 3151019
Abstract
PROCHECK
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