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PDBsum entry 3cwg
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Transcription
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PDB id
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3cwg
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References listed in PDB file
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Key reference
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Title
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Crystal structure of unphosphorylated stat3 core fragment.
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Authors
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Z.Ren,
X.Mao,
C.Mertens,
R.Krishnaraj,
J.Qin,
P.K.Mandal,
M.J.Romanowski,
J.S.Mcmurray,
X.Chen.
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Ref.
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Biochem Biophys Res Commun, 2008,
374,
1-5.
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PubMed id
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Abstract
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Signal transducers and activators of transcription (STATs) are latent
cytoplasmic transcriptional factors that play an important role in cytokine and
growth factor signaling. Here we report a 3.05 A-resolution crystal structure of
an unphosphorylated STAT3 core fragment. The overall monomeric structure is very
similar to that of the phosphorylated STAT3 core fragment. However, the dimer
interface observed in the unphosphorylated STAT1 core fragment structure is
absent in the STAT3 structure. Solution studies further demonstrate that the
core fragment of STAT3 is primarily monomeric. Mutations corresponding to those
in STAT1, which lead to disruption of the core fragment interface and prolonged
tyrosine phosphorylation, show little or no effect on the tyrosine
phosphorylation kinetics of STAT3. These results highlight the structural and
biochemical differences between STAT3 and STAT1, and suggest different
regulation mechanisms of these two proteins.
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