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UniProt functional annotation for Q8ZNF9 | ||
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| UniProt code: Q8ZNF9. |
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| Organism: | |
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Salmonella. |
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| Function: | |
Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy- L-rhamnonate (KDR). Can also dehydrate L-lyxonate and L-mannonate, although less efficiently, but not 2-keto-4-hydroxyheptane-1,7-dioate. {ECO:0000269|PubMed:18754693}. |
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| Catalytic activity: | |
Reaction=L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O; Xref=Rhea:RHEA:23080, ChEBI:CHEBI:15377, ChEBI:CHEBI:58118, ChEBI:CHEBI:58371; EC=4.2.1.90; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:18754693}; Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000269|PubMed:18754693}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.25 mM for L-rhamnonate {ECO:0000269|PubMed:18754693}; KM=1.6 mM for L-lyxonate {ECO:0000269|PubMed:18754693}; KM=0.06 mM for L-mannonate {ECO:0000269|PubMed:18754693}; Note=The catalytic efficiency observed with L-rhamnonate as substrate is 533- and 8-fold higher than that observed with L-lyxonate and L- mannonate, respectively.; |
| Subunit: | |
Homooctamer; tetramer of dimers. {ECO:0000269|PubMed:18754693}. |
| Miscellaneous: | |
Reaction proceeds via a syn dehydration. |
| Similarity: | |
Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.