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* Residue conservation analysis
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Immunity
27:900-911
(2007)
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PubMed id:
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The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition.
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L.C.Sullivan,
C.S.Clements,
T.Beddoe,
D.Johnson,
H.L.Hoare,
J.Lin,
T.Huyton,
E.J.Hopkins,
H.H.Reid,
M.C.Wilce,
J.Kabat,
F.Borrego,
J.E.Coligan,
J.Rossjohn,
A.G.Brooks.
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ABSTRACT
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The CD94-NKG2 receptor family that regulates NK and T cells is unique among the
lectin-like receptors encoded within the natural killer cell complex. The
function of the CD94-NKG2 receptors is dictated by the pairing of the invariant
CD94 polypeptide with specific NKG2 isoforms to form a family of functionally
distinct heterodimeric receptors. However, the structural basis for this
selective pairing and how they interact with their ligand, HLA-E, is unknown. We
describe the 2.5 A resolution crystal structure of CD94-NKG2A in which the mode
of dimerization contrasts with that of other homodimeric NK receptors. Despite
structural homology between the CD94 and NKG2A subunits, the dimer interface is
asymmetric, thereby providing a structural basis for the preferred heterodimeric
assembly. Structure-based sequence comparisons of other CD94-NKG2 family
members, combined with extensive mutagenesis studies on HLA-E and CD94-NKG2A,
allows a model of the interaction between CD94-NKG2A and HLA-E to be
established, in which the invariant CD94 chain plays a more dominant role in
interacting with HLA-E in comparison to the variable NKG2 chain.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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K.Higai,
C.Suzuki,
Y.Imaizumi,
X.Xin,
Y.Azuma,
and
K.Matsumoto
(2011).
Binding affinities of NKG2D and CD94 to sialyl Lewis X-expressing N-glycans and heparin.
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Biol Pharm Bull,
34,
8.
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T.Beddoe,
A.G.Brooks,
and
J.Rossjohn
(2011).
How opposites attract.
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Immunol Cell Biol,
89,
163-164.
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X.Xin,
K.Higai,
Y.Imaizumi,
C.Suzuki,
K.Ito,
A.Itoh,
S.Matsumoto,
Y.Azuma,
and
K.Matsumoto
(2011).
Natural killer group 2A (NKG2A) and natural killer group 2C (NKG2C) bind to sulfated glycans and α2,3-NeuAc-containing glycoproteins.
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Biol Pharm Bull,
34,
480-485.
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J.W.Leavenworth,
C.Schellack,
H.J.Kim,
L.Lu,
P.Spee,
and
H.Cantor
(2010).
Analysis of the cellular mechanism underlying inhibition of EAE after treatment with anti-NKG2A F(ab')2.
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Proc Natl Acad Sci U S A,
107,
2562-2567.
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M.E.Call,
and
J.J.Chou
(2010).
A view into the blind spot: solution NMR provides new insights into signal transduction across the lipid bilayer.
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Structure,
18,
1559-1569.
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M.T.Orr,
J.Wu,
M.Fang,
L.J.Sigal,
P.Spee,
T.Egebjerg,
E.Dissen,
S.Fossum,
J.H.Phillips,
and
L.L.Lanier
(2010).
Development and function of CD94-deficient natural killer cells.
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PLoS One,
5,
e15184.
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N.G.Walpole,
L.Kjer-Nielsen,
L.Kostenko,
J.McCluskey,
A.G.Brooks,
J.Rossjohn,
and
C.S.Clements
(2010).
The structure and stability of the monomorphic HLA-G are influenced by the nature of the bound peptide.
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J Mol Biol,
397,
467-480.
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PDB codes:
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T.H.Nguyen,
L.C.Sullivan,
T.C.Kotsimbos,
A.P.Schwarer,
and
N.A.Mifsud
(2010).
Cross-presentation of HCMV chimeric protein enables generation and measurement of polyclonal T cells.
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Immunol Cell Biol,
88,
676-684.
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B.K.Kaiser,
J.C.Pizarro,
J.Kerns,
and
R.K.Strong
(2008).
Structural basis for NKG2A/CD94 recognition of HLA-E.
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Proc Natl Acad Sci U S A,
105,
6696-6701.
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PDB code:
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E.J.Petrie,
C.S.Clements,
J.Lin,
L.C.Sullivan,
D.Johnson,
T.Huyton,
A.Heroux,
H.L.Hoare,
T.Beddoe,
H.H.Reid,
M.C.Wilce,
A.G.Brooks,
and
J.Rossjohn
(2008).
CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence.
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J Exp Med,
205,
725-735.
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PDB code:
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L.C.Sullivan,
C.S.Clements,
J.Rossjohn,
and
A.G.Brooks
(2008).
The major histocompatibility complex class Ib molecule HLA-E at the interface between innate and adaptive immunity.
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Tissue Antigens,
72,
415-424.
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S.L.Rogers,
and
J.Kaufman
(2008).
High allelic polymorphism, moderate sequence diversity and diversifying selection for B-NK but not B-lec, the pair of lectin-like receptor genes in the chicken MHC.
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Immunogenetics,
60,
461-475.
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W.Held,
and
R.A.Mariuzza
(2008).
Cis interactions of immunoreceptors with MHC and non-MHC ligands.
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Nat Rev Immunol,
8,
269-278.
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Y.T.Bryceson,
and
E.O.Long
(2008).
Line of attack: NK cell specificity and integration of signals.
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Curr Opin Immunol,
20,
344-352.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
