PDBsum entry 3b76

Ligase

PDB id: 3b76

Contents

Protein chain

101 a.a. *

Ligands

EDO ×2

Metals

_NA

Waters ×124

* Residue conservation analysis

PDB id:

3b76

Name:

Ligase

Title:

Crystal structure of the third pdz domain of human ligand-of-numb protein-x (lnx1) in complex with thE C-terminal peptide from the coxsackievirus and adenovirus receptor

Structure:

E3 ubiquitin-protein ligase lnx. Chain: a, b. Fragment: third pdz domain: residues 504-594. Synonym: numb-binding protein 1, ligand of numb-protein x 1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: lnx1, lnx. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.75Å R-factor: 0.177 R-free: 0.205

Authors:

E.Ugochukwu,N.Burgess-Brown,G.Berridge,J.Elkins,G.Bunkoczi, A.C.W.Pike,M.Sundstrom,C.H.Arrowsmith,J.Weigelt,A.M.Edwards, O.Gileadi,F.Von Delft,D.Doyle,Structural Genomics Consortium (Sgc)

Key ref:

E.Ugochukwu et al. Crystal structure of the third pdz domain of human ligand-Of-Numb protein-X (lnx1) in complex with the c-Terminal peptide from the coxsackievirus and adenovirus receptor.. To be published, .

Date:

30-Oct-07 Release date: 13-Nov-07

Protein chains

Q8TBB1  (LNX1_HUMAN) -  E3 ubiquitin-protein ligase LNX from Homo sapiens

Seq: 728 a.a.

Struc: 101 a.a.*

* PDB and UniProt seqs differ at 10 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine