PDBsum entry 3b1l

Ligase

PDB id: 3b1l

Contents

Protein chain

76 a.a.

Waters ×33

PDB id:

3b1l

Name:

Ligase

Title:

Crystal structure of parkin ubiquitin-like domain r33q mutant

Structure:

E3 ubiquitin-protein ligase parkin. Chain: x. Fragment: ubiquitin-like domain. Engineered: yes. Mutation: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.85Å R-factor: 0.191 R-free: 0.243

Authors:

K.Tomoo,A.Ikemiya,Y.Amami,Y.In,T.Ishida

Key ref:

K.Tomoo et al. Crystal structure of parkin ubiquitin-Like domain r33 mutant. To be published, .

Date:

04-Jul-11 Release date: 04-Jul-12

Protein chain

Q9WVS6  (PRKN_MOUSE) -  E3 ubiquitin-protein ligase parkin from Mus musculus

Seq: 464 a.a.

Struc: 76 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.2.31 - RBR-type E3 ubiquitin transferase.
• Reaction: [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N₆-ubiquitinyl-L-lysine