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PDBsum entry 3b0a
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Signaling protein/metal binding protein
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PDB id
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3b0a
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References listed in PDB file
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Key reference
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Title
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Specific recognition of linear ubiquitin chains by the npl4 zinc finger (nzf) domain of the hoil-1l subunit of the linear ubiquitin chain assembly complex.
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Authors
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Y.Sato,
H.Fujita,
A.Yoshikawa,
M.Yamashita,
A.Yamagata,
S.E.Kaiser,
K.Iwai,
S.Fukai.
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Ref.
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Proc Natl Acad Sci U S A, 2011,
108,
20520-20525.
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PubMed id
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Abstract
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The linear ubiquitin chain assembly complex (LUBAC) is a key nuclear factor-κB
(NF-κB) pathway component that produces linear polyubiquitin chains. The
HOIL-1L subunit of LUBAC has been shown to bind linear chains; however, detailed
structural and functional analyses on the binding between LUBAC and linear
chains have not been performed. In this study, we found that the Npl4 zinc
finger (NZF) domain of HOIL-1L specifically binds linear polyubiquitin chains
and determined the crystal structure of the HOIL-1L NZF domain in complex with
linear diubiquitin at 1.7-Å resolution. The HOIL-1L NZF domain consists of a
zinc-coordinating "NZF core" region and an additional α-helical
"NZF tail" region. The HOIL-1L NZF core binds both the canonical
Ile44-centered hydrophobic surface on the distal ubiquitin and a Phe4-centered
hydrophobic patch on the proximal ubiquitin, representing a mechanism for the
specific recognition of linear chains. The NZF tail binds the proximal ubiquitin
to enhance the binding affinity. These recognition mechanisms were supported by
the accompanying in vitro and in vivo structure-based mutagenesis experiments.
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