PDBsum entry 3aoi

Transcription, transferase/DNA/RNA

PDB id

3aoi

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Contents

			Protein chains

					(+ 0 more) 223 a.a. *


					1083 a.a. *


					1316 a.a. *


					93 a.a. *


					154 a.a. *

			DNA/RNA

			Metals

					_MG ×3


					_ZN ×3

* Residue conservation analysis

PDB id:

3aoi

Links

Name:

Transcription, transferase/DNA/RNA

Title:

RNA polymerase-gfh1 complex (crystal type 2)

Structure:

DNA-directed RNA polymerase subunit alpha. Chain: a, b, f, g, k, l. Synonym: rnap subunit alpha, transcriptase subunit alpha, RNA polymerase subunit alpha. DNA-directed RNA polymerase subunit beta. Chain: c, h, m. Synonym: rnap subunit beta, transcriptase subunit beta, RNA polymerase subunit beta. DNA-directed RNA polymerase subunit beta'.

Source:

Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Synthetic: yes. Other_details: DNA oligomer. Other_details: RNA oligomer. Gene: ttha1042. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

4.30Å

R-factor:

0.317

R-free:

0.338

Authors:

S.Tagami,S.Sekine,T.Kumarevel,M.Yamamoto,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

S.Tagami et al. (2010). Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein. Nature, 468, 978-982. PubMed id: 21124318

Date:

30-Sep-10

Release date:

08-Dec-10

PROCHECK

	Headers

	References

Protein chains

Q5SHR6 (RPOA_THET8) - DNA-directed RNA polymerase subunit alpha from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: Struc:					315 a.a. 223 a.a.

Protein chains

Q8RQE9 (RPOB_THET8) - DNA-directed RNA polymerase subunit beta from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: Struc:

Seq: Struc:

Seq: Struc:					1119 a.a. 1083 a.a.

Protein chains

Q8RQE8 (RPOC_THET8) - DNA-directed RNA polymerase subunit beta' from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					1524 a.a. 1316 a.a.

Protein chains

Q8RQE7 (RPOZ_THET8) - DNA-directed RNA polymerase subunit omega from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: Struc:					99 a.a. 93 a.a.

Protein chains

Q5SJG6 (GFH1_THET8) - Transcription inhibitor protein Gfh1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)

Seq: Struc:					156 a.a. 154 a.a.

Key:

PfamA domain

Secondary structure

DNA/RNA chains

		C-C-G-C-C-G-C 7 bases
		G-C-G-G-C-G-G 7 bases
		G-C-C-G-C-A 6 bases
		U-G-C-G-G-C-G-G 8 bases
		G-C-C-G-C 5 bases
		G-C-G-G-C-G-G 7 bases



		Enzyme reactions

Enzyme class:

Chains A, B, C, D, E, F, G, H, I, J, K, L, M, N, O: E.C.2.7.7.6 - DNA-directed Rna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

RNA(n)	+	ribonucleoside 5'-triphosphate	=	RNA(n+1)	+	diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Nature 468:978-982 (2010)
PubMed id: 21124318

Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein.
S.Tagami, S.Sekine, T.Kumarevel, N.Hino, Y.Murayama, S.Kamegamori, M.Yamamoto, K.Sakamoto, S.Yokoyama.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22334143

L.Davis, and J.W.Chin (2012).
Designer proteins: applications of genetic code expansion in cell biology.

Nat Rev Mol Cell Biol, 13, 168-182.

21386817

F.W.Martinez-Rucobo, S.Sainsbury, A.C.Cheung, and P.Cramer (2011).
Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity.

EMBO J, 30, 1302-1310.

PDB code:

3qqc

21468097

V.Svetlov, and E.Nudler (2011).
Clamping the clamp of RNA polymerase.

EMBO J, 30, 1190-1191.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.