PDBsum entry 3aji

Chaperone/protein binding

PDB id: 3aji

Contents

Protein chains

229 a.a. *

73 a.a. *

Waters ×327

* Residue conservation analysis

PDB id:

3aji

Name:

Chaperone/protein binding

Title:

Structure of gankyrin-s6atpase photo-cross-linked site-specifically, and incoporated by genetic code expansion

Structure:

26s proteasome non-atpase regulatory subunit 10. Chain: a, c. Synonym: 26s proteasome regulatory subunit p28, gankyrin. Engineered: yes. Mutation: yes. Proteasome (prosome, macropain) 26s subunit, atpase, 4. Chain: b, d. Fragment: c-terminal domain. Synonym: s6c.

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: psmd10. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: psmc4. Expression_system_taxid: 562

Resolution:

2.05Å R-factor: 0.171 R-free: 0.229

Authors:

S.Sato,S.Mimasu,A.Sato,N.Hino,K.Sakamoto,T.Umehara,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

S.Sato et al. (2011). Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid. Biochemistry, 50, 250-257. PubMed id: 21128684

Date:

07-Jun-10 Release date: 22-Dec-10

Protein chains

Q9Z2X2  (PSD10_MOUSE) -  26S proteasome non-ATPase regulatory subunit 10 from Mus musculus

Seq: 231 a.a.

Struc: 229 a.a.*

Protein chains

P54775  (PRS6B_MOUSE) -  26S proteasome regulatory subunit 6B from Mus musculus

Seq: 418 a.a.

Struc: 73 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Biochemistry 50:250-257 (2011)

PubMed id: 21128684

Crystallographic study of a site-specifically cross-linked protein complex with a genetically incorporated photoreactive amino acid.

S.Sato, S.Mimasu, A.Sato, N.Hino, K.Sakamoto, T.Umehara, S.Yokoyama.

ABSTRACT

No abstract given.