UniProt functional annotation for Q60610



	UniProt functional annotation for Q60610

UniProt code: Q60610.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration. {ECO:0000250|UniProtKB:Q13009}.

Subunit: Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1 (By similarity). Interacts with BAIAP2. Interacts (via PDZ domain) with CNTNAP4, SDC1 and SDC3 (via C- terminus) (By similarity). Interacts with CD44, PARD3 and MAPK8IP2. Interacts with EPHA8; regulates clathrin-mediated endocytosis of EPHA8. Interacts with NTRK2; mediates the activation of RAC1 by BDNF. {ECO:0000250, ECO:0000269|PubMed:11130063, ECO:0000269|PubMed:16801538, ECO:0000269|PubMed:19893486, ECO:0000269|PubMed:20496116}.

Subcellular location: Cell junction {ECO:0000269|PubMed:17339315}. Cell membrane {ECO:0000269|PubMed:17339315}; Peripheral membrane protein {ECO:0000269|PubMed:17339315}; Cytoplasmic side {ECO:0000269|PubMed:17339315}. Note=Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (By similarity). Detected at the boundary between cells with actin-rich protrusions. {ECO:0000250}.

Tissue specificity: Highly expressed in brain and testis and at low or moderate levels in almost all other normal tissues. Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.

Domain: The first PH domain mediates interaction with membranes enriched in phosphoinositides. {ECO:0000269|PubMed:17339315}.

Ptm: Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell. {ECO:0000250|UniProtKB:Q13009}.

Similarity: Belongs to the TIAM family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration. {ECO:0000250\|UniProtKB:Q13009}.


Subunit:		Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1 (By similarity). Interacts with BAIAP2. Interacts (via PDZ domain) with CNTNAP4, SDC1 and SDC3 (via C- terminus) (By similarity). Interacts with CD44, PARD3 and MAPK8IP2. Interacts with EPHA8; regulates clathrin-mediated endocytosis of EPHA8. Interacts with NTRK2; mediates the activation of RAC1 by BDNF. {ECO:0000250, ECO:0000269\|PubMed:11130063, ECO:0000269\|PubMed:16801538, ECO:0000269\|PubMed:19893486, ECO:0000269\|PubMed:20496116}.
Subcellular location:		Cell junction {ECO:0000269\|PubMed:17339315}. Cell membrane {ECO:0000269\|PubMed:17339315}; Peripheral membrane protein {ECO:0000269\|PubMed:17339315}; Cytoplasmic side {ECO:0000269\|PubMed:17339315}. Note=Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (By similarity). Detected at the boundary between cells with actin-rich protrusions. {ECO:0000250}.
Tissue specificity:		Highly expressed in brain and testis and at low or moderate levels in almost all other normal tissues. Found in virtually all analyzed tumor cell lines including B- and T-lymphomas, neuroblastomas, melanomas and carcinomas.
Domain:		The first PH domain mediates interaction with membranes enriched in phosphoinositides. {ECO:0000269\|PubMed:17339315}.
Ptm:		Ubiquitinated. Undergoes 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 by a CUL3(KBTBD6/7) E3 ubiquitin ligase complex composed of CUL3, RBX1, KBTBD6 and KBTBD7. 'Lys-48' ubiquitination at Lys-1404 and Lys-1420 triggers proteasomal degradation. Ubiquitination at Lys-1404 and Lys-1420 by CUL3(KBTBD6/7) also requires the membrane-associated protein GABARAP and may therefore be spatially restricted within the cell. {ECO:0000250\|UniProtKB:Q13009}.
Similarity:		Belongs to the TIAM family. {ECO:0000305}.