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PDBsum entry 3a02
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Gene regulation
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PDB id
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3a02
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References listed in PDB file
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Key reference
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Title
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Cooperative DNA-Binding and sequence-Recognition mechanism of aristaless and clawless.
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Authors
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K.Miyazono,
Y.Zhi,
Y.Takamura,
K.Nagata,
K.Saigo,
T.Kojima,
M.Tanokura.
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Ref.
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Embo J, 2010,
29,
1613-1623.
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PubMed id
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Abstract
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To achieve accurate gene regulation, some homeodomain proteins bind
cooperatively to DNA to increase those site specificities. We report a ternary
complex structure containing two homeodomain proteins, aristaless (Al) and
clawless (Cll), bound to DNA. Our results show that the extended conserved
sequences of the Cll homeodomain are indispensable to cooperative DNA binding.
In the Al-Cll-DNA complex structure, the residues in the extended regions are
used not only for the intermolecular contacts between the two homeodomain
proteins but also for the sequence-recognition mechanism of DNA by direct
interactions. The residues in the extended N-terminal arm lie within the minor
groove of DNA to form direct interactions with bases, whereas the extended
conserved region of the C-terminus of the homeodomain interacts with Al to
stabilize and localize the third alpha helix of the Cll homeodomain. This
structure suggests a novel mode for the cooperativity of homeodomain proteins.
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