PDBsum entry 3vhq

Hydrolase

PDB id

3vhq

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Contents

			Protein chain

					385 a.a.

			Metals

					_CA ×5

			Waters ×263

PDB id:

3vhq

Links

Name:

Hydrolase

Title:

Crystal structure of the ca6 site mutant of pro-sa-subtilisin

Structure:

Tk-subtilisin. Chain: a. Engineered: yes. Mutation: yes

Source:

Thermococcus kodakarensis. Organism_taxid: 69014. Strain: kod1. Gene: tk1675. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.15Å

R-factor:

0.175

R-free:

0.241

Authors:

R.Uehara,Y.Takeuchi,S.Tanaka,H.Matsumura,Y.Koga,K.Takano,S.Kanaya

Key ref:

R.Uehara et al. (2012). Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis. Biochemistry, 51, 5369-5378. PubMed id: 22686281

Date:

01-Sep-11

Release date:

11-Jul-12

PROCHECK

	Headers

	References

Protein chain

P58502 (TKSU_THEKO) - Tk-subtilisin from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)

Seq: Struc:					422 a.a. 385 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 2 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

E.C.3.4.21.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Biochemistry 51:5369-5378 (2012)
PubMed id: 22686281

Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis.
R.Uehara, Y.Takeuchi, S.Tanaka, K.Takano, Y.Koga, S.Kanaya.

ABSTRACT

No abstract given.