PDBsum entry 3uou

Hydrolase/hydrolase inhibitor

PDB id: 3uou

Contents

Protein chains

240 a.a.

54 a.a.

Ligands

SO4 ×8

GOL ×7

Waters ×206

PDB id:

3uou

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of the kunitz-type protease inhibitor shpi-1 lys13leu mutant in complex with pancreatic elastase

Structure:

Chymotrypsin-like elastase family member 1. Chain: a. Fragment: peptidase s1 domain. Synonym: elastase-1. Kunitz-type proteinase inhibitor shpi-1. Chain: b. Fragment: kunitz-type proteinase inhibitor shpi-1. Engineered: yes. Mutation: yes

Source:

Sus scrofa. Pigs,swine,wild boar. Organism_taxid: 9823. Other_details: pancreas. Stichodactyla helianthus. Carribean sea anemone. Organism_taxid: 6123. Expressed in: pichia pastoris. Expression_system_taxid: 4922.

Resolution:

2.00Å R-factor: 0.168 R-free: 0.212

Authors:

R.Garcia-Fernandez,M.Perbandt,D.Rehders,Y.Gonzalez-Gonzalez, M.A.Chavez,C.Betzel,L.Redecke

Key ref:

R.García-Fernández et al. (2015). Three-dimensional Structure of a Kunitz-type Inhibitor in Complex with an Elastase-like Enzyme. J Biol Chem, 290, 14154-14165. PubMed id: 25878249 DOI: 10.1074/jbc.M115.647586

Date:

17-Nov-11 Release date: 21-Nov-12

Protein chain

P00772  (CELA1_PIG) -  Chymotrypsin-like elastase family member 1 from Sus scrofa

Seq: 266 a.a.

Struc: 240 a.a.

Protein chain

P31713  (VKT1_STIHL) -  PI-stichotoxin-She2a from Stichodactyla helianthus

Seq: 55 a.a.

Struc: 54 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.21.36 - pancreatic elastase.
• Reaction: Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.

DOI no: 10.1074/jbc.M115.647586

J Biol Chem 290:14154-14165 (2015)

PubMed id: 25878249

Three-dimensional Structure of a Kunitz-type Inhibitor in Complex with an Elastase-like Enzyme.

R.García-Fernández, M.Perbandt, D.Rehders, P.Ziegelmüller, N.Piganeau, U.Hahn, C.Betzel, M.d.e. .L.Chávez, L.Redecke.

ABSTRACT

No abstract given.