PDBsum entry 3trs

Hydrolase

PDB id: 3trs

Contents

Protein chains

37 a.a.

171 a.a.

30 a.a.

Ligands

DMS ×2

Waters ×198

PDB id:

3trs

Name:

Hydrolase

Title:

The crystal structure of aspergilloglutamic peptidase from aspergillus niger

Structure:

Aspergillopepsin-2 light chain. Chain: a, c. Synonym: aspergillopepsin ii light chain. Aspergillopepsin-2 heavy chain. Chain: b, d. Synonym: aspergillopepsin ii heavy chain. Ec: 3.4.23.19

Source:

Aspergillus niger. Organism_taxid: 29838. Strain: var. Macrosporus. Strain: var. Macrosporus

Resolution:

1.60Å R-factor: 0.210 R-free: 0.239

Authors:

H.Sasaki,K.Kubota,W.C.Lee,J.Ohtsuka,M.Kojima,K.Takahashi,M.Tanokura

Key ref:

H.Sasaki et al. (2012). The crystal structure of an intermediate dimer of aspergilloglutamic peptidase that mimics the enzyme-activation product complex produced upon autoproteolysis. J Biochem (tokyo), 152, 45-52. PubMed id: 22569035

Date:

10-Sep-11 Release date: 22-Aug-12

Protein chain

P24665  (PRTA_ASPNG) -  Aspergillopepsin-2 from Aspergillus niger

Seq: 282 a.a.

Struc: 37 a.a.

Protein chains

P24665  (PRTA_ASPNG) -  Aspergillopepsin-2 from Aspergillus niger

Seq: 282 a.a.

Struc: 171 a.a.

Protein chain

P24665  (PRTA_ASPNG) -  Aspergillopepsin-2 from Aspergillus niger

Seq: 282 a.a.

Struc: 30 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.4.23.19 - aspergillopepsin Ii.
• Reaction: Preferential cleavage in A chain of insulin: 3-Asn-|-Gln-4, 13-Gly-|-Ala-14, and 26-Tyr-|-Thr-27.

J Biochem (tokyo) 152:45-52 (2012)

PubMed id: 22569035

The crystal structure of an intermediate dimer of aspergilloglutamic peptidase that mimics the enzyme-activation product complex produced upon autoproteolysis.

H.Sasaki, K.Kubota, W.C.Lee, J.Ohtsuka, M.Kojima, S.Iwata, A.Nakagawa, K.Takahashi, M.Tanokura.

ABSTRACT

No abstract given.