PDBsum entry 3tc2

Hydrolase inhibitor

PDB id: 3tc2

Contents

Protein chains

181 a.a.

Waters ×501

PDB id:

3tc2

Name:

Hydrolase inhibitor

Title:

Crystal structure of potato serine protease inhibitor.

Structure:

Kunitz-type proteinase inhibitor p1h5. Chain: a, b, c. Fragment: unp residues 21-221

Source:

Solanum tuberosum. Potatoes. Organism_taxid: 4113. Strain: cv elkana

Resolution:

1.60Å R-factor: 0.171 R-free: 0.221

Authors:

E.M.Meulenbroek,E.A.J.Thomassen,N.S.Pannu

Key ref:

E.M.Meulenbroek et al. (2012). Structure of a post-translationally processed heterodimeric double-headed Kunitz-type serine protease inhibitor from potato. Acta Crystallogr D Biol Crystallogr, 68, 794-799. PubMed id: 22751664

Date:

08-Aug-11 Release date: 20-Jun-12

Protein chains

Q8S380  (Q8S380_SOLTU) -  Kunitz-type inhibitor B from Solanum tuberosum

Seq: 221 a.a.

Struc: 181 a.a.

Acta Crystallogr D Biol Crystallogr 68:794-799 (2012)

PubMed id: 22751664

Structure of a post-translationally processed heterodimeric double-headed Kunitz-type serine protease inhibitor from potato.

E.M.Meulenbroek, E.A.Thomassen, L.Pouvreau, J.P.Abrahams, H.Gruppen, N.S.Pannu.

ABSTRACT

Potato serine protease inhibitor (PSPI) constitutes about 22% of the total amount of proteins in potato tubers (cv. Elkana), making it the most abundant protease inhibitor in the plant. PSPI is a heterodimeric double-headed Kunitz-type serine protease inhibitor that can tightly and simultaneously bind two serine proteases by mimicking the substrate of the enzyme with its reactive-site loops. Here, the crystal structure of PSPI is reported, representing the first heterodimeric double-headed Kunitz-type serine protease inhibitor structure to be determined. PSPI has a β-trefoil fold and, based on the structure, two reactive-site loops bearing residues Phe75 and Lys95 were identified.