PDBsum entry 3qj3

Hydrolase

PDB id: 3qj3

Contents

Protein chains

319 a.a.

Ligands

ACT ×4

Waters ×530

PDB id:

3qj3

Name:

Hydrolase

Title:

Structure of digestive procathepsin l2 proteinase from tenebrio molitor larval midgut

Structure:

Cathepsin l-like protein. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Tenebrio molitor. Yellow mealworm beetle. Organism_taxid: 7067. Gene: pcal2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.85Å R-factor: 0.186 R-free: 0.231

Authors:

D.Beton,C.R.Guzzo,W.R.Terra,C.S.Farah

Key ref:

D.Beton et al. (2012). The 3D structure and function of digestive cathepsin L-like proteinases of Tenebrio molitor larval midgut. Insect Biochem Mol Biol, 42, 655-664. PubMed id: 22659439

Date:

28-Jan-11 Release date: 01-Feb-12

Protein chains

Q69G21  (Q69G21_TENMO) -  C1 family cathepsin L5 from Tenebrio molitor

Seq: 336 a.a.

Struc: 319 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.22.15 - cathepsin L.
• Reaction: Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Insect Biochem Mol Biol 42:655-664 (2012)

PubMed id: 22659439

The 3D structure and function of digestive cathepsin L-like proteinases of Tenebrio molitor larval midgut.

D.Beton, C.R.Guzzo, A.F.Ribeiro, C.S.Farah, W.R.Terra.

ABSTRACT

No abstract given.