PDBsum entry 3oha

Transferase/DNA

PDB id: 3oha

Contents

Protein chain

516 a.a. *

DNA/RNA

Ligands

DCP

SO4 ×3

Metals

_MG ×2

Waters ×705

* Residue conservation analysis

PDB id:

3oha

Name:

Transferase/DNA

Title:

Yeast DNA polymerase eta inserting dctp opposite an 8oxog lesion

Structure:

DNA polymerase eta. Chain: a. Fragment: unp residues 1-513. Synonym: radiation-sensitive protein 30. Engineered: yes. Mutation: yes. 5'-d( Gp Tp Cp Cp Tp Cp Cp Cp Cp Tp (Doc))-3'. Chain: p. Engineered: yes.

Source:

Saccharomyces cerevisiae. Brewer's yeast,lager beer yeast,yeast. Organism_taxid: 4932. Gene: dbh1, rad30, ydr419w. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes

Resolution:

2.00Å R-factor: 0.159 R-free: 0.195

Authors:

T.D.Silverstein,R.Jain,A.K.Aggarwal

Key ref:

T.D.Silverstein et al. (2010). Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase η. Structure, 18, 1463-1470. PubMed id: 21070945

Date:

17-Aug-10 Release date: 22-Dec-10

Protein chain

Q04049  (POLH_YEAST) -  DNA polymerase eta from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 632 a.a.

Struc: 516 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

G-T-C-C-T-C-C-C-C-T-DOC 11 bases

T-8OG-G-A-G-G-G-G-A-G-G-A-C 13 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Structure 18:1463-1470 (2010)

PubMed id: 21070945

Structural basis for error-free replication of oxidatively damaged DNA by yeast DNA polymerase η.

T.D.Silverstein, R.Jain, R.E.Johnson, L.Prakash, S.Prakash, A.K.Aggarwal.

ABSTRACT

No abstract given.