PDBsum entry 3noo

Lyase

PDB id

3noo

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Contents

			Protein chains

					228 a.a. *

			Ligands

					EDO ×7


					ACT ×2

			Waters ×444

* Residue conservation analysis

PDB id:

3noo

Links

Name:

Lyase

Title:

Crystal structure of c101a isocyanide hydratase from pseudomonas fluorescens

Structure:

Thij/pfpi family protein. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Pseudomonas fluorescens. Organism_taxid: 294. Gene: pfl_4109. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.03Å

R-factor:

0.130

R-free:

0.166

Authors:

M.Lakshminarasimhan,P.Madzelan,R.Nan,N.M.Milkovic,M.A.Wilson

Key ref:

M.Lakshminarasimhan et al. (2010). Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase. J Biol Chem, 285, 29651-29661. PubMed id: 20630867

Date:

25-Jun-10

Release date:

07-Jul-10

PROCHECK

	Headers

	References

Protein chains

Q4K977 (Q4K977_PSEF5) - Isonitrile hydratase InhA from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

Seq: Struc:					228 a.a. 228 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.4.2.1.103 - cyclohexyl-isocyanide hydratase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

N-cyclohexylformamide = cyclohexyl isocyanide + H2O

N-cyclohexylformamide	=	cyclohexyl isocyanide	+	H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	J Biol Chem 285:29651-29661 (2010)
PubMed id: 20630867

Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.
M.Lakshminarasimhan, P.Madzelan, R.Nan, N.M.Milkovic, M.A.Wilson.

ABSTRACT

No abstract given.