PDBsum entry 3non

Lyase

PDB id: 3non

Contents

Protein chains

226 a.a. *

Ligands

EDO ×5

Waters ×496

* Residue conservation analysis

PDB id:

3non

Name:

Lyase

Title:

Crystal structure of isocyanide hydratase from pseudomonas fluorescens

Structure:

Isocyanide hydratase. Chain: a, b. Engineered: yes

Source:

Pseudomonas fluorescens. Bacterium fluorescen. Organism_taxid: 294. Gene: pfl_4109. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.05Å R-factor: 0.139 R-free: 0.169

Authors:

M.Lakshminarasimhan,P.Madzelan,R.Nan,N.M.Milkovic,M.A.Wilson

Key ref:

M.Lakshminarasimhan et al. (2010). Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase. J Biol Chem, 285, 29651-29661. PubMed id: 20630867

Date:

25-Jun-10 Release date: 14-Jul-10

Protein chains

Q4K977  (Q4K977_PSEF5) -  Isonitrile hydratase InhA from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

Seq: 228 a.a.

Struc: 226 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.2.1.103 - cyclohexyl-isocyanide hydratase.
• Reaction: N-cyclohexylformamide = cyclohexyl isocyanide + H2O

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

J Biol Chem 285:29651-29661 (2010)

PubMed id: 20630867

Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.

M.Lakshminarasimhan, P.Madzelan, R.Nan, N.M.Milkovic, M.A.Wilson.

ABSTRACT

No abstract given.