PDBsum entry 3nae

Transferase/DNA

PDB id: 3nae

Contents

Protein chain

903 a.a. *

DNA/RNA

Ligands

DTP

Metals

_CA ×4

Waters ×704

* Residue conservation analysis

PDB id:

3nae

Name:

Transferase/DNA

Title:

Rb69 DNA polymerase (y567a) ternary complex with datp opposite guanidinohydantoin

Structure:

DNA polymerase. Chain: a. Synonym: gp43. Engineered: yes. Mutation: yes. DNA (5'-d( Tp Cp Ap (G35) p Gp Tp Ap Ap Gp Cp Ap Gp Tp Cp Cp Gp Cp G)-3'). Chain: t. Engineered: yes.

Source:

Enterobacteria phage rb69. Bacteriophage rb69. Organism_taxid: 12353. Gene: 43, gp43. Expressed in: escherichia coli. Expression_system_taxid: 511693. Synthetic: yes. Synthetic: yes

Resolution:

2.00Å R-factor: 0.196 R-free: 0.231

Authors:

M.Wang,J.Beckman,G.Blaha,J.Wang,W.H.Konigsberg

Key ref:

J.Beckman et al. (2010). Substitution of Ala for Tyr567 in RB69 DNA polymerase allows dAMP and dGMP to be inserted opposite Guanidinohydantoin . Biochemistry, 49, 8554-8563. PubMed id: 20795733

Date:

01-Jun-10 Release date: 15-Sep-10

Protein chain

Q38087  (DPOL_BPR69) -  DNA-directed DNA polymerase from Escherichia phage RB69

Seq: 903 a.a.

Struc: 903 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

T-C-A-G35-G-T-A-A-G-C-A-G-T-C-C-G-C-G 18 bases

G-C-G-G-A-C-T-G-C-T-T-A-DOC 13 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Biochemistry 49:8554-8563 (2010)

PubMed id: 20795733

Substitution of Ala for Tyr567 in RB69 DNA polymerase allows dAMP and dGMP to be inserted opposite Guanidinohydantoin .

J.Beckman, M.Wang, G.Blaha, J.Wang, W.H.Konigsberg.

ABSTRACT

No abstract given.