PDBsum entry 3mwi

Hydrolase

PDB id: 3mwi

Contents

Protein chain

246 a.a.

Ligands

B25

SO4 ×2

Waters ×144

PDB id:

3mwi

Name:

Hydrolase

Title:

The complex crystal structure of urokianse and 5-nitro-1h-indole-2- amidine

Structure:

Urokinase-type plasminogen activator. Chain: u. Fragment: c-terminal domain, unp residues 179-424. Synonym: u-plasminogen activator, upa, urokinase-type plasminogen activator chain b. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: human. Expressed in: pichia pastoris. Expression_system_taxid: 4922.

Resolution:

2.03Å R-factor: 0.219 R-free: 0.297

Authors:

L.G.Jiang,H.Y.Yu,C.Yuan,Z.X.Huang,M.D.Huang

Key ref:

L.G.Jiang et al. The complex crystal structure of urokianse and 5-Nitro-1h-Indole-2-Amidine. To be published, .

Date:

06-May-10 Release date: 01-Jun-11

Protein chain

P00749  (UROK_HUMAN) -  Urokinase-type plasminogen activator from Homo sapiens

Seq: 431 a.a.

Struc: 246 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.21.73 - u-plasminogen activator.
• Reaction: Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.