PDBsum entry 3mp2

Hydrolase

PDB id: 3mp2

Contents

Protein chain

211 a.a. *

Metals

_ZN

Waters ×173

* Residue conservation analysis

PDB id:

3mp2

Name:

Hydrolase

Title:

Crystal structure of transmissible gastroenteritis virus papain-like protease 1

Structure:

Non-structural protein 3. Chain: a. Fragment: unp residues 1071-1281. Synonym: papain-like protease 1. Engineered: yes

Source:

Porcine transmissible gastroenteritis coronavirus. Tgev. Organism_taxid: 11151. Strain: purdue. Gene: 1a. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å R-factor: 0.176 R-free: 0.223

Authors:

J.A.Wojdyla,I.Manolaridis,P.A.Tucker

Key ref:

J.A.Wojdyla et al. (2010). Papain-like protease 1 from transmissible gastroenteritis virus: crystal structure and enzymatic activity toward viral and cellular substrates. J Virol, 84, 10063-10073. PubMed id: 20668092

Date:

24-Apr-10 Release date: 08-Sep-10

Protein chain

P0C6V2  (R1A_CVPPU) -  Replicase polyprotein 1a from Porcine transmissible gastroenteritis coronavirus (strain Purdue)

Seq: 4017 a.a.

Struc: 211 a.a.

Enzyme reactions

• Enzyme class 1: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
• Enzyme class 2: E.C.3.4.22.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

J Virol 84:10063-10073 (2010)

PubMed id: 20668092

Papain-like protease 1 from transmissible gastroenteritis virus: crystal structure and enzymatic activity toward viral and cellular substrates.

J.A.Wojdyla, I.Manolaridis, P.B.van Kasteren, M.Kikkert, E.J.Snijder, A.E.Gorbalenya, P.A.Tucker.

ABSTRACT

No abstract given.