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PDBsum entry 3keh

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3keh
Jmol
Contents
Protein chain
497 a.a. *
Ligands
NDG
SO4 ×8
GOL ×2
NAG ×2
NAG-NAG
Waters ×42
* Residue conservation analysis
PDB id:
3keh
Name: Hydrolase
Title: Crystal structure of n370s glucocerebrosidase mutant at ph 7
Structure: Glucocerebrosidase. Chain: a, b. Synonym: beta-glucocerebrosidase, acid beta-glucosidase, d- n-acylsphingosine glucohydrolase, alglucerase, imiglucerase engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: gba, gc, gluc. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108
Resolution:
2.80Å     R-factor:   0.215     R-free:   0.273
Authors: R.R.Wei,S.Boucher,C.Q.Pan,T.Edmunds
Key ref: R.R.Wei et al. Crystal structure of glucocerebrosidase containing th mutation: implication on chaperon therapy. To be published, .
Date:
26-Oct-09     Release date:   27-Oct-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P04062  (GLCM_HUMAN) -  Glucosylceramidase
Seq:
Struc:
 
Seq:
Struc:
536 a.a.
497 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.2.1.45  - Glucosylceramidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine
D-glucosyl-N-acylsphingosine
+ H(2)O
=
D-glucose
Bound ligand (Het Group name = NDG)
matches with 62.00% similarity
+ N-acylsphingosine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   5 terms 
  Biological process     metabolic process   23 terms 
  Biochemical function     protein binding     5 terms