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PDBsum entry 3k8o

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protein ligands Protein-protein interface(s) links
Transferase PDB id
3k8o
Jmol
Contents
Protein chain
(+ 0 more) 283 a.a. *
Ligands
SO4 ×16
229 ×6
Waters ×150
* Residue conservation analysis
PDB id:
3k8o
Name: Transferase
Title: Crystal structure of human purine nucleoside phosphorylase i with datme-immh
Structure: Purine nucleoside phosphorylase. Chain: e, q, s, t, u, y. Synonym: pnp, inosine phosphorylase. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: np, pnp. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.40Å     R-factor:   0.226     R-free:   0.272
Authors: M.Ho,A.Rinaldo-Matthis,S.C.Almo,V.L.Schramm
Key ref: M.Ho et al. Crystal structure of human purine nucleoside phosphorylase in complex with datme-Immucillin h. Proc.Natl.Acad.Sci.Usa, .
Date:
14-Oct-09     Release date:   02-Mar-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00491  (PNPH_HUMAN) -  Purine nucleoside phosphorylase
Seq:
Struc:
289 a.a.
283 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.2.4.2.1  - Purine-nucleoside phosphorylase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
2. Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Purine nucleoside
Bound ligand (Het Group name = 229)
matches with 42.31% similarity
+ phosphate
= purine
+ alpha-D-ribose 1-phosphate
Purine deoxynucleoside
+ phosphate
= purine
+ 2'-deoxy-alpha-D-ribose 1-phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     intracellular   5 terms 
  Biological process     small molecule metabolic process   16 terms 
  Biochemical function     catalytic activity     9 terms