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PDBsum entry 3i40

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protein Protein-protein interface(s) links
Hormone PDB id
3i40
Jmol
Contents
Protein chains
21 a.a.
30 a.a. *
Waters ×35
* Residue conservation analysis
PDB id:
3i40
Name: Hormone
Title: Human insulin
Structure: Insulin a chain. Chain: a. Engineered: yes. Insulin b chain. Chain: b. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Resolution:
1.85Å     R-factor:   0.202     R-free:   0.228
Authors: V.I.Timofeev,V.V.Bezuglov,K.A.Miroshnikov,R.N.Chuprov-Netoch I.P.Kuranova
Key ref: V.I.Timofeev et al. (2010). X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid. Acta Crystallogr Sect F Struct Biol Cryst Commun, 66, 259-263. PubMed id: 20208155
Date:
01-Jul-09     Release date:   05-Jan-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P01308  (INS_HUMAN) -  Insulin
Seq:
Struc:
110 a.a.
21 a.a.
Protein chain
Pfam   ArchSchema ?
P01308  (INS_HUMAN) -  Insulin
Seq:
Struc:
110 a.a.
30 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biochemical function     hormone activity     1 term  

 

 
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:259-263 (2010)
PubMed id: 20208155  
 
 
X-ray investigation of gene-engineered human insulin crystallized from a solution containing polysialic acid.
V.I.Timofeev, R.N.Chuprov-Netochin, V.R.Samigina, V.V.Bezuglov, K.A.Miroshnikov, I.P.Kuranova.
 
  ABSTRACT  
 
Attempts to crystallize the noncovalent complex of recombinant human insulin with polysialic acid were carried out under normal and microgravity conditions. Both crystal types belonged to the same space group, I2(1)3, with unit-cell parameters a = b = c = 77.365 A, alpha = beta = gamma = 90.00 degrees. The reported space group and unit-cell parameters are almost identical to those of cubic insulin reported in the PDB. The results of X-ray studies confirmed that the crystals obtained were cubic insulin crystals and that they contained no polysialic acid or its fragments. Electron-density maps were calculated using X-ray diffraction sets from earth-grown and microgravity-grown crystals and the three-dimensional structure of the insulin molecule was determined and refined. The conformation and secondary-structural elements of the insulin molecule in different crystal forms were compared.