PDBsum entry 3ffi

Transferase

PDB id: 3ffi

Contents

Protein chains

549 a.a. *

403 a.a. *

Ligands

3OB

Waters ×60

* Residue conservation analysis

PDB id:

3ffi

Name:

Transferase

Title:

HIV-1 rt with pyridone non-nucleoside inhibitor

Structure:

Reverse transcriptase/ribonuclease h. Chain: a. Fragment: unp residues 588-1148. Synonym: p66 rt. Engineered: yes. Rt p51. Chain: b. Fragment: unp residues 588-1027. Engineered: yes

Source:

HIV-1 m:b_hxb2r. HIV-1. Organism_taxid: 11706. Gene: gag-pol. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.60Å R-factor: 0.237 R-free: 0.285

Authors:

S.F.Harris,A.Villasenor

Key ref:

J.Kennedy-Smith and z.k.sweeney Pyridone diaryl ether non-Nucleoside inhibitors of HIV-1 reverse transcriptase. To be published, .

Date:

03-Dec-08 Release date: 15-Dec-09

Protein chain

P04585  (POL_HV1H2) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)

Seq: 1435 a.a.

Struc: 549 a.a.

Protein chain

P04585  (POL_HV1H2) -  Gag-Pol polyprotein from Human immunodeficiency virus type 1 group M subtype B (isolate HXB2)

Seq: 1435 a.a.

Struc: 403 a.a.

Enzyme reactions

• Enzyme class 1: Chains A, B: E.C.2.7.7.- - ?????
• Enzyme class 2: Chains A, B: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: Chains A, B: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 4: Chains A, B: E.C.3.1.-.-
• Enzyme class 5: Chains A, B: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 6: Chains A, B: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 7: Chains A, B: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site