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Immune system PDB-id
 3cfe
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213 a.a. *
219 a.a. *
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SO4 ×5
GOL ×3

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PDB id: 3cfe
Name: Immune system
Title: Crystal structure of purple-fluorescent antibody ep2-25c10

Structure:		 Purple-fluorescent antibody ep2-25c10-kappa light chain. Chain: l, a. Fragment: fab. Purple-fluorescent antibody ep2-25c10-igg2b heavy chain. Chain: h, b. Fragment: fab

Source: 		Mus musculus. House mouse. Organism_taxid: 10090. Cell: hybridoma cells from 129gix+ spleen cells fused with balb/c myeloma cells. Other_details: purified from ascitic fluid. Other_details: purified from ascitic fluid

Resolution: 		2.99Å

R-factor: 		0.213

R-free: 		0.255

Authors: 		E.W.Debler,A.Heine,I.A.Wilson

Key ref: 		E.W.Debler et al. (2008). Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.. Science, 319, 1232-1235. [PubMed id: 18309081] [DOI: 10.1126/science.1153445]

Date: 		03-Mar-08

Release date: 		18-Mar-08

Related entries: 		1fl3
crystal structure of blue fluorescent antibody (19g2) in
complex with stilbene hapten at 277k
1ub5
crystal structure of antibody 19g2 with hapten at 100k
1ub6
crystal structure of antibody 19g2 with sera ligand
3cfb
high-resolution structure of blue fluorescent antibody ep2-
19g2 in complex with stilbene hapten at 100k
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    Key reference    
 
 
DOI no: 10.1126/science.1153445 Science 319:1232-1235 (2008)
PubMed id: 18309081  
 
 
Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.
E.W.Debler, G.F.Kaufmann, M.M.Meijler, A.Heine, J.M.Mee, G.Pljevaljcic, A.J.Di Bilio, P.G.Schultz, D.P.Millar, K.D.Janda, I.A.Wilson, H.B.Gray, R.A.Lerner.
 
  ABSTRACT  
 
The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.
 

Literature references that cite this PDB file's key reference
  PubMed id Reference
19606431 C.N.Falco, K.M.Dykstra, B.P.Yates, and P.B.Berget (2009).
scFv-based fluorogen activating proteins and variable domain inhibitors as fluorescent biosensor platforms.
  Biotechnol J, 4, 1328-1336.  
19597589 Y.Hong, J.W.Lam, and B.Z.Tang (2009).
Aggregation-induced emission: phenomenon, mechanism and applications.
  Chem Commun (Camb), 0, 4332-4353.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.