PDBsum entry: 3cfb

Immune system

PDB-id: 3cfb

Contents

Description

Header details

Header records

References

PROCHECK

Protein chains

218 a.a. *

213 a.a. *

Ligands

SPB ×2

GOL ×6

Waters ×590

* Residue conservation analysis

Tools

Clefts Calculation

• PDB id: 3cfb
• Name: Immune system
• Title: High-resolution structure of blue fluorescent antibody ep2- 19g2 in complex with stilbene hapten at 100k
• Structure: Blue fluorescent antibody ep2-19g2-kappa light chain. Chain: l, a. Fragment: fab. Blue fluorescent antibody ep2-19g2-igg2b heavy chain. Chain: h, b. Fragment: fab
• Source: Mus musculus. House mouse. Organism_taxid: 10090. Cell: hybridoma cells from 129gix+ spleen cells fused with balb/c myeloma cells. Other_details: purified from ascitic fluid. Other_details: purified from ascitic fluid
• Resolution: 1.60Å
• R-factor: 0.187
• R-free: 0.218
• Authors: E.W.Debler,I.A.Wilson
• Key ref: E.W.Debler et al. (2008). Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.. Science, 319, 1232-1235. [PubMed id: 18309081] [DOI: 10.1126/science.1153445]
• Date: 03-Mar-08
• Release date: 18-Mar-08
• Related entries: 1fl3 ; crystal structure of blue fluorescent antibody (19g2) in ; complex with stilbene hapten at 277k ; 1ub5 ; crystal structure of antibody 19g2 with hapten at 100k ; 1ub6 ; crystal structure of antibody 19g2 with sera ligand ; 3cfc ; high-resolution structure of blue fluorescent antibody ep2- ; 19g2 ; ... plus others (see Header records)

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Surface

Key reference

DOI no: 10.1126/science.1153445

Science 319:1232-1235 (2008)

PubMed id: 18309081

Deeply inverted electron-hole recombination in a luminescent antibody-stilbene complex.

E.W.Debler, G.F.Kaufmann, M.M.Meijler, A.Heine, J.M.Mee, G.Pljevaljcic, A.J.Di Bilio, P.G.Schultz, D.P.Millar, K.D.Janda, I.A.Wilson, H.B.Gray, R.A.Lerner.

ABSTRACT

The blue-emissive antibody EP2-19G2 that has been elicited against trans-stilbene has unprecedented ability to produce bright luminescence and has been used as a biosensor in various applications. We show that the prolonged luminescence is not stilbene fluorescence. Instead, the emissive species is a charge-transfer excited complex of an anionic stilbene and a cationic, parallel pi-stacked tryptophan. Upon charge recombination, this complex generates exceptionally bright blue light. Complex formation is enabled by a deeply penetrating ligand-binding pocket, which in turn results from a noncanonical interface between the two variable domains of the antibody.