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PDBsum entry 3a5m

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protein ligands metals Protein-protein interface(s) links
Contractile protein PDB id
3a5m
Jmol
Contents
Protein chains
123 a.a. *
365 a.a. *
Ligands
ATP
Metals
_CA ×2
_MG
Waters ×358
* Residue conservation analysis
PDB id:
3a5m
Name: Contractile protein
Title: Crystal structure of a dictyostelium p109i mg2+-actin in com human gelsolin segment 1
Structure: Gelsolin. Chain: s. Fragment: gelsolin-like 1, residues 53-176. Synonym: actin-depolymerizing factor, adf, brevin, agel. Engineered: yes. Mutation: yes. Major actin. Chain: c. Synonym: actin-1, actin-2, actin-2-sub 1, actin-4, actin-5,
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Dictyostelium discoideum. Slime mold. Organism_taxid: 44689. Expressed in: dictyostelium.
Resolution:
2.40Å     R-factor:   0.182     R-free:   0.223
Authors: K.Murakami,T.Yasunaga,T.Q.Noguchi,T.Q.Uyeda,T.Wakabayashi
Key ref: K.Murakami et al. (2010). Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release. Cell, 143, 275-287. PubMed id: 20946985
Date:
09-Aug-09     Release date:   27-Oct-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06396  (GELS_HUMAN) -  Gelsolin
Seq:
Struc:
 
Seq:
Struc:
782 a.a.
123 a.a.*
Protein chain
Pfam   ArchSchema ?
P07830  (ACT1_DICDI) -  Major actin
Seq:
Struc:
376 a.a.
365 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cell leading edge   11 terms 
  Biological process     macropinocytosis   10 terms 
  Biochemical function     nucleotide binding     6 terms  

 

 
Cell 143:275-287 (2010)
PubMed id: 20946985  
 
 
Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.
K.Murakami, T.Yasunaga, T.Q.Noguchi, Y.Gomibuchi, K.X.Ngo, T.Q.Uyeda, T.Wakabayashi.
 
  ABSTRACT  
 
No abstract given.