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PDBsum entry 2zrs
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Contents |
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* Residue conservation analysis
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PDB id:
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Apoptosis
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Title:
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Crystal structure of ca2+-bound form of des3-23alg-2
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Structure:
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Programmed cell death protein 6. Chain: a, b, c, d, e, f, g, h. Fragment: unp residues 24-191. Synonym: apoptosis-linked gene 2 protein, calcium-binding protein alg-2. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: pdcd6, alg2. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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3.10Å
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R-factor:
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0.228
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R-free:
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0.280
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Authors:
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H.Suzuki,M.Kawasaki,T.Kakiuchi,H.Shibata,S.Wakatsuki,M.Maki
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Key ref:
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H.Suzuki
et al.
(2008).
Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2.
Acta Crystallogr Sect F Struct Biol Cryst Commun,
64,
974-977.
PubMed id:
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Date:
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01-Sep-08
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Release date:
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04-Nov-08
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PROCHECK
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Headers
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References
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O75340
(PDCD6_HUMAN) -
Programmed cell death protein 6 from Homo sapiens
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Seq:
Struc:
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191 a.a.
165 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Acta Crystallogr Sect F Struct Biol Cryst Commun
64:974-977
(2008)
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PubMed id:
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Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2.
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H.Suzuki,
M.Kawasaki,
T.Kakiuchi,
H.Shibata,
S.Wakatsuki,
M.Maki.
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ABSTRACT
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ALG-2 (apoptosis-linked gene 2) is an apoptosis-linked calcium-binding protein
with five EF-hand motifs in the C-terminal region. N-terminally truncated ALG-2
(des3-23ALG-2) was crystallized by the vapour-diffusion method in buffer
consisting of either 50 mM MES pH 6.5, 12.5%(v/v) 2-propanol and 150 mM calcium
acetate or 100 mM MES pH 6.0, 15%(v/v) ethanol and 200 mM zinc acetate. Crystals
of the Ca(2+)-bound form belonged to space group P2(1)2(1)2(1), with unit-cell
parameters a = 54.8, b = 154.4, c = 237.7 A, alpha = beta = gamma = 90 degrees ,
and diffracted to 3.1 A resolution. Crystals of the Zn(2+)-bound form belonged
to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.8, b = 147.5, c =
230.7 A, alpha = beta = gamma = 90 degrees , and diffracted to 3.3 A resolution.
The structures of the Ca(2+)-bound form and the Zn(2+)-bound form were solved by
the molecular-replacement method. Although both crystals contained eight ALG-2
molecules per asymmetric unit, the metal-ion locations and octameric
arrangements were found to be significantly different.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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T.Inuzuka,
H.Suzuki,
M.Kawasaki,
H.Shibata,
S.Wakatsuki,
and
M.Maki
(2010).
Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2DeltaGF122) and structural roles of F122 in target recognition.
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BMC Struct Biol,
10,
25.
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PDB codes:
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so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
