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UniProt functional annotation for O73946 | ||
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| UniProt code: O73946. |
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| Organism: | |
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
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| Function: | |
DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks. Unwinds the lagging strand from forked DNA structures in a 3'-5' direction. PCNA, the DNA polymerase sliding clamp subunit, stimulates the helicase activity, and may alter substrate specificity. Unwinds branched DNA (Holliday junctions) in an ATP-dependent fashion; ss- and dsDNA stimulate ATPase to the greatest extent, although it preferentially binds DNA with a single-stranded region. Processes a RecA-mediated recombination intermediate between gapped circular and homologous linear dsDNA. {ECO:0000255|HAMAP-Rule:MF_00442, ECO:0000269|PubMed:15677450, ECO:0000269|PubMed:16436047}. |
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| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; Evidence={ECO:0000255|HAMAP-Rule:MF_00442}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15677450}; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:15677450}; Note=Divalent cations, Mg(2+) and Zn(2+) are best. {ECO:0000269|PubMed:15677450}; |
| Subunit: | |
Monomer. Interacts with PCNA. {ECO:0000255|HAMAP- Rule:MF_00442, ECO:0000269|PubMed:15677450, ECO:0000269|PubMed:16436047}. |
| Induction: | |
Constitutively expressed (at protein level). {ECO:0000269|PubMed:15677450}. |
| Similarity: | |
Belongs to the helicase family. Hel308 subfamily. {ECO:0000255|HAMAP-Rule:MF_00442}. |
Annotations taken from UniProtKB at the EBI.