PDBsum entry 2zf0

Blood clotting/hydrolase inhibitor

PDB id: 2zf0

Contents

Protein chains

28 a.a. *

251 a.a. *

11 a.a. *

Ligands

51U

Metals

_NA ×2

Waters ×174

* Residue conservation analysis

PDB id:

2zf0

Name:

Blood clotting/hydrolase inhibitor

Title:

Exploring thrombin s1 pocket

Structure:

Thrombin light chain. Chain: l. Synonym: coagulation factor ii. Thrombin heavy chain. Chain: h. Synonym: coagulation factor ii. Hirudin variant-1. Chain: i. Fragment: unp residues 54-64.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Tissue: blood plasma. Synthetic: yes. Hirudo medicinalis. Organism_taxid: 6421. Other_details: synthetic fragment of hirudin from hirudo medicinalis

Resolution:

2.20Å R-factor: 0.174 R-free: 0.267

Authors:

B.Baum,A.Heine,G.Klebe

Key ref:

B.Baum et al. Exploring thrombin s1 pocket. To be published, .

Date:

18-Dec-07 Release date: 04-Nov-08

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 28 a.a.

Protein chain

P00734  (THRB_HUMAN) -  Prothrombin from Homo sapiens

Seq: 622 a.a.

Struc: 251 a.a.

Protein chain

P01050  (HIRV1_HIRME) -  Hirudin variant-1 from Hirudo medicinalis

Seq: 65 a.a.

Struc: 11 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains L, H: E.C.3.4.21.5 - thrombin.
• Reaction: Preferential cleavage: Arg-|-Gly; activates fibrinogen to fibrin and releases fibrinopeptide A and B.