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PDBsum entry 2zcw
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Transcription
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PDB id
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2zcw
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References listed in PDB file
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Key reference
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Title
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Global gene expression mediated by thermus thermophilus sdrp, A crp/fnr family transcriptional regulator.
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Authors
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Y.Agari,
A.Kashihara,
S.Yokoyama,
S.Kuramitsu,
A.Shinkai.
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Ref.
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Mol Microbiol, 2008,
70,
60-75.
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PubMed id
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Abstract
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Thermus thermophilus SdrP is one of four cyclic AMP receptor protein
(CRP)/fumarate and nitrate reduction regulator (FNR) family proteins from the
extremely thermophilic bacterium T. thermophilus HB8. Expression of sdrP mRNA
increased in the stationary phase during cultivation at 70 degrees C. Although
the sdrP gene was non-essential, an sdrP-deficient strain showed growth defects,
particularly when grown in a synthetic medium, and increased sensitivity to
disulphide stress. The expression of several genes was altered in the sdrP
disruptant. Among them, we found eight SdrP-dependent promoters using in vitro
transcription assays. A predicted SdrP binding site similar to that recognized
by Escherichia coli CRP was found upstream of each SdrP-dependent promoter. In
the wild-type strain, expression of these eight genes tended to increase upon
entry into the stationary phase. Transcriptional activation in vitro was
independent of any added effector molecule. The hypothesis that apo-SdrP is the
active form of the protein was supported by the observation that the
three-dimensional structure of apo-SdrP is similar to that of the DNA-binding
form of E. coli CRP. Based on the properties of the SdrP-regulated genes found
in this study, it is speculated that SdrP is involved in nutrient and energy
supply, redox control, and polyadenylation of mRNA.
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