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PDBsum entry 2zcw
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Transcription
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PDB id
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2zcw
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Contents |
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* Residue conservation analysis
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Mol Microbiol
70:60-75
(2008)
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PubMed id:
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Global gene expression mediated by Thermus thermophilus SdrP, a CRP/FNR family transcriptional regulator.
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Y.Agari,
A.Kashihara,
S.Yokoyama,
S.Kuramitsu,
A.Shinkai.
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ABSTRACT
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Thermus thermophilus SdrP is one of four cyclic AMP receptor protein
(CRP)/fumarate and nitrate reduction regulator (FNR) family proteins from the
extremely thermophilic bacterium T. thermophilus HB8. Expression of sdrP mRNA
increased in the stationary phase during cultivation at 70 degrees C. Although
the sdrP gene was non-essential, an sdrP-deficient strain showed growth defects,
particularly when grown in a synthetic medium, and increased sensitivity to
disulphide stress. The expression of several genes was altered in the sdrP
disruptant. Among them, we found eight SdrP-dependent promoters using in vitro
transcription assays. A predicted SdrP binding site similar to that recognized
by Escherichia coli CRP was found upstream of each SdrP-dependent promoter. In
the wild-type strain, expression of these eight genes tended to increase upon
entry into the stationary phase. Transcriptional activation in vitro was
independent of any added effector molecule. The hypothesis that apo-SdrP is the
active form of the protein was supported by the observation that the
three-dimensional structure of apo-SdrP is similar to that of the DNA-binding
form of E. coli CRP. Based on the properties of the SdrP-regulated genes found
in this study, it is speculated that SdrP is involved in nutrient and energy
supply, redox control, and polyadenylation of mRNA.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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K.Sakamoto,
Y.Agari,
K.Agari,
S.Kuramitsu,
and
A.Shinkai
(2010).
Structural and functional characterization of the transcriptional repressor CsoR from Thermus thermophilus HB8.
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Microbiology,
156,
1993-2005.
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PDB code:
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Y.Agari,
S.Kuramitsu,
and
A.Shinkai
(2010).
Identification of novel genes regulated by the oxidative stress-responsive transcriptional activator SdrP in Thermus thermophilus HB8.
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FEMS Microbiol Lett,
313,
127-134.
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D.T.Gallagher,
N.Smith,
S.K.Kim,
H.Robinson,
and
P.T.Reddy
(2009).
Profound Asymmetry in the Structure of the cAMP-free cAMP Receptor Protein (CRP) from Mycobacterium tuberculosis.
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J Biol Chem,
284,
8228-8232.
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S.Mesa,
L.Reutimann,
H.M.Fischer,
and
H.Hennecke
(2009).
Posttranslational control of transcription factor FixK2, a key regulator for the Bradyrhizobium japonicum-soybean symbiosis.
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Proc Natl Acad Sci U S A,
106,
21860-21865.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
