PDBsum entry 2ysf

Protein binding

PDB id: 2ysf

Contents

Protein chain

40 a.a. *

* Residue conservation analysis

PDB id:

2ysf

Name:

Protein binding

Title:

Solution structure of the fourth ww domain from the human e3 ubiquitin-protein ligase itchy homolog, itch

Structure:

E3 ubiquitin-protein ligase itchy homolog. Chain: a. Fragment: ww domain, unp residues 480-512. Synonym: itch, atrophin-1-interacting protein 4, aip4, nfe2- associated polypeptide 1, napp1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: itch. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

S.Ohnishi,H.Li,S.Koshiba,T.Harada,S.Watanabe,T.Kigawa,S.Yokoyama, Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

S.Ohnishi et al. Solution structure of the fourth ww domain from the human e3 ubiquitin-Protein ligase itchy homolog, Itch. To be published, .

Date:

03-Apr-07 Release date: 09-Oct-07

Protein chain

Q96J02  (ITCH_HUMAN) -  E3 ubiquitin-protein ligase Itchy homolog from Homo sapiens

Seq: 903 a.a.

Struc: 40 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine