PDBsum entry 2y8i

Motor protein

PDB id: 2y8i

Contents

Protein chain

758 a.a.

Ligands

ADP

Metals

_MG

Waters ×258

PDB id:

2y8i

Name:

Motor protein

Title:

Structural basis for the allosteric interference of myosin function by mutants g680a and g680v of dictyostelium myosin-2

Structure:

Myosin-2 heavy chain. Chain: x. Fragment: motor domain, residues 2-759. Synonym: myosin ii heavy chain. Engineered: yes. Mutation: yes

Source:

Dictyostelium discoideum. Slime mold. Organism_taxid: 44689. Expressed in: dictyostelium discoideum. Expression_system_taxid: 44689.

Resolution:

3.13Å R-factor: 0.249 R-free: 0.337

Authors:

M.Preller,S.Bauer,N.Adamek,S.Fujita-Becker,R.Fedorov,M.A.Geeves, D.J.Manstein

Key ref:

M.Preller et al. (2011). Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V. J Biol Chem, 286, 35051-35060. PubMed id: 21841195

Date:

07-Feb-11 Release date: 20-Jul-11

Protein chain

P08799  (MYS2_DICDI) -  Myosin-2 heavy chain from Dictyostelium discoideum

Seq: 2116 a.a.

Struc: 758 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.6.4.1 - Transferred entry: 5.6.1.8.
• Reaction: ATP + H₂O = ADP + phosphate

ATP + H(2)O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Biol Chem 286:35051-35060 (2011)

PubMed id: 21841195

Structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V.

M.Preller, S.Bauer, N.Adamek, S.Fujita-Becker, R.Fedorov, M.A.Geeves, D.J.Manstein.

ABSTRACT

No abstract given.