PDBsum entry 2y6i

Hydrolase/inhibitor

PDB id: 2y6i

Contents

Protein chain

671 a.a.

Ligands

IPI-GLY-PRO-ALA

FLC

TRS ×3

PEG ×2

1PE

Metals

_ZN

Waters ×44

PDB id:

2y6i

Name:

Hydrolase/inhibitor

Title:

Crystal structure of collagenase g from clostridium histolyticum in complex with isoamylphosphonyl-gly-pro-ala at 3.25 angstrom resolution

Structure:

Collagenase. Chain: a. Fragment: residues 119-880. Synonym: collagenase g. Engineered: yes. Isoamylphosphonyl-gly-pro-ala. Chain: b. Engineered: yes

Source:

Clostridium histolyticum. Organism_taxid: 1498. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630

Resolution:

3.25Å R-factor: 0.219 R-free: 0.266

Authors:

U.Eckhard,H.Brandstetter

Key ref:

U.Eckhard et al. (2011). Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis. Nat Struct Biol, 18, 1109-1114. PubMed id: 21947205

Date:

21-Jan-11 Release date: 28-Sep-11

Protein chain

Q9X721  (COLG_HATHI) -  Collagenase ColG from Hathewaya histolytica

Seq: 1118 a.a.

Struc: 671 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.3 - microbial collagenase.
• Reaction: Digestion of native collagen in the triple helical region at Xaa-|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'.
• Cofactor: Zn(2+)

Nat Struct Biol 18:1109-1114 (2011)

PubMed id: 21947205

Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis.

U.Eckhard, E.Schönauer, D.Nüss, H.Brandstetter.

ABSTRACT

No abstract given.