PDBsum entry 2y0b

Hydrolase/protein binding

PDB id: 2y0b

Contents

Protein chains

141 a.a.

94 a.a.

119 a.a.

Ligands

MPD ×2

MRD ×2

Waters ×345

PDB id:

2y0b

Name:

Hydrolase/protein binding

Title:

Caspase-3 in complex with an inhibitory darpin-3.4_s76r

Structure:

Caspase-3 subunit p17. Chain: a, c. Synonym: caspase-3, casp-3, apopain, cysteine protease cpp32, cpp-32, protein yama, srebp cleavage activity 1, sca-1. Engineered: yes. Caspase-3. Chain: b, d. Synonym: caspase-3, casp-3, apopain, cysteine protease cpp32, cpp-32, protein yama, srebp cleavage activity 1, sca-1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: t7 promoter expression. Synthetic construct. Organism_taxid: 32630. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.193 R-free: 0.217

Authors:

J.Barandun,T.Schroeder,P.Mittl,M.G.Grutter

Key ref:

T.Schroeder et al. (2013). Specific inhibition of caspase-3 by a competitive DARPin: molecular mimicry between native and designed inhibitors. Structure, 21, 277-289. PubMed id: 23333429

Date:

01-Dec-10 Release date: 21-Dec-11

Protein chains

P42574  (CASP3_HUMAN) -  Caspase-3 from Homo sapiens

Seq: 277 a.a.

Struc: 141 a.a.*

Protein chains

P42574  (CASP3_HUMAN) -  Caspase-3 from Homo sapiens

Seq: 277 a.a.

Struc: 94 a.a.

Protein chains

No UniProt id for this chain

Struc: 119 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.4.22.56 - caspase-3.

Structure 21:277-289 (2013)

PubMed id: 23333429

Specific inhibition of caspase-3 by a competitive DARPin: molecular mimicry between native and designed inhibitors.

T.Schroeder, J.Barandun, A.Flütsch, C.Briand, P.R.Mittl, M.G.Grütter.

ABSTRACT

No abstract given.