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PDBsum entry 2xzl
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Hydrolase/RNA
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PDB id
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2xzl
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PDB id:
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| Name: |
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Hydrolase/RNA
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Title:
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Upf1-RNA complex
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Structure:
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Atp-dependent helicase nam7. Chain: a. Fragment: ch domain and helicase domain, residues 54-850. Synonym: up frameshift factor 1, nonsense-mediated mRNA decay protein 1, nuclear accomodation of mitochondria 7 protein, up-frameshift suppressor 1. Engineered: yes. 5- r( Up Up Up Up Up Up Up Up U) -3. Chain: b.
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Source:
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Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630
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Resolution:
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2.40Å
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R-factor:
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0.200
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R-free:
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0.243
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Authors:
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S.Chakrabarti,U.Jayachandran,F.Bonneau,F.Fiorini,C.Basquin,S.Domcke, H.Le Hir,E.Conti
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Key ref:
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S.Chakrabarti
et al.
(2011).
Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.
Mol Cell,
41,
693-703.
PubMed id:
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Date:
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26-Nov-10
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Release date:
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30-Mar-11
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PROCHECK
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Headers
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References
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P30771
(NAM7_YEAST) -
ATP-dependent helicase NAM7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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Seq:
Struc:
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971 a.a.
756 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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U-U-U-U-U-U-U-U-U
9 bases
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Enzyme class 1:
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E.C.3.6.4.12
- Dna helicase.
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Reaction:
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ATP + H2O = ADP + phosphate + H+
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ATP
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+
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H2O
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=
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ADP
Bound ligand (Het Group name = )
corresponds exactly
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+
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phosphate
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+
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H(+)
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Enzyme class 2:
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E.C.3.6.4.13
- Rna helicase.
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Reaction:
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ATP + H2O = ADP + phosphate + H+
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ATP
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+
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H2O
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=
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ADP
Bound ligand (Het Group name = )
corresponds exactly
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+
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phosphate
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+
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H(+)
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Mol Cell
41:693-703
(2011)
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PubMed id:
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Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.
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S.Chakrabarti,
U.Jayachandran,
F.Bonneau,
F.Fiorini,
C.Basquin,
S.Domcke,
H.Le Hir,
E.Conti.
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ABSTRACT
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.J.Shoemaker,
and
R.Green
(2012).
Translation drives mRNA quality control.
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Nat Struct Mol Biol,
19,
594-601.
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I.Barbosa,
N.Haque,
F.Fiorini,
C.Barrandon,
C.Tomasetto,
M.Blanchette,
and
H.Le Hir
(2012).
Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
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Nat Struct Mol Biol,
19,
983-990.
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R.Melero,
G.Buchwald,
R.Castaño,
M.Raabe,
D.Gil,
M.Lázaro,
H.Urlaub,
E.Conti,
and
O.Llorca
(2012).
The cryo-EM structure of the UPF-EJC complex shows UPF1 poised toward the RNA 3' end.
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Nat Struct Mol Biol,
19,
498.
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S.Kervestin,
and
A.Jacobson
(2012).
NMD: a multifaceted response to premature translational termination.
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Nat Rev Mol Cell Biol,
13,
700-712.
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M.L.Gleghorn,
and
L.E.Maquat
(2011).
UPF1 learns to relax and unwind.
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Mol Cell,
41,
621-623.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
