PDBsum entry 2xxf

Oxidoreductase

PDB id: 2xxf

Contents

Protein chains

335 a.a.

Ligands

PEG ×7

MES ×4

PG4 ×2

Metals

_ZN ×6

_CU ×4

Waters ×614

PDB id:

2xxf

Name:

Oxidoreductase

Title:

Cu metallated h254f mutant of nitrite reductase

Structure:

Dissimilatory copper-containing nitrite reductase. Chain: a, b. Fragment: residues 26-360. Synonym: nitrite reductase, nir. Engineered: yes. Mutation: yes

Source:

Achromobacter xylosoxidans. Organism_taxid: 85698. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.50Å R-factor: 0.146 R-free: 0.177

Authors:

M.A.Hough,R.R.Eady,S.S.Hasnain

Key ref:

N.G.Leferink et al. (2011). Proton-coupled electron transfer in the catalytic cycle of Alcaligenes xylosoxidans copper-dependent nitrite reductase. Biochemistry, 50, 4121-4131. PubMed id: 21469743

Date:

10-Nov-10 Release date: 18-May-11

Supersedes:

2jl3

Protein chains

O68601  (O68601_ALCXX) -  Copper-containing nitrite reductase from Alcaligenes xylosoxydans xylosoxydans

Seq: 360 a.a.

Struc: 335 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.1.7.2.1 - nitrite reductase (NO-forming).
• Reaction: nitric oxide + Fe(III)-[cytochrome c] + H2O = Fe(II)-[cytochrome c] + nitrite + 2 H⁺
• Cofactor: Cu cation or Fe cation; FAD

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 50:4121-4131 (2011)

PubMed id: 21469743

Proton-coupled electron transfer in the catalytic cycle of Alcaligenes xylosoxidans copper-dependent nitrite reductase.

N.G.Leferink, C.Han, S.V.Antonyuk, D.J.Heyes, S.E.Rigby, M.A.Hough, R.R.Eady, N.S.Scrutton, S.S.Hasnain.

ABSTRACT

No abstract given.