PDBsum entry 2xxa

Protein transport

PDB id

2xxa

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Contents

			Protein chains

					414 a.a. *


					283 a.a. *

			DNA/RNA

			Ligands

					GCP ×4

			Metals

					_MG ×4

			Waters ×12

* Residue conservation analysis

PDB id:

2xxa

Links

Name:

Protein transport

Title:

The crystal structure of the signal recognition particle (srp) in complex with its receptor(sr)

Structure:

Signal recognition particle protein. Chain: a, c. Fragment: residues 1-433. Synonym: fifty-four homolog, p48. Engineered: yes. Mutation: yes. Srp receptor ftsy. Chain: b, d. Fragment: residues 196-497.

Source:

Escherichia coli k-12. Organism_taxid: 83333. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: plyss. Synthetic: yes. Deinococcus radiodurans. Organism_taxid: 1299

Resolution:

3.94Å

R-factor:

0.233

R-free:

0.267

Authors:

S.F.Ataide,N.Schmitz,K.Shen,A.Ke,S.Shan,J.A.Doudna,N.Ban

Key ref:

S.F.Ataide et al. (2011). The crystal structure of the signal recognition particle in complex with its receptor. Science, 331, 881-886. PubMed id: 21330537

Date:

09-Nov-10

Release date:

02-Mar-11

PROCHECK

	Headers

	References

Protein chains

P0AGD7 (SRP54_ECOLI) - Signal recognition particle protein from Escherichia coli (strain K12)

Seq: Struc:					453 a.a. 414 a.a.*

Protein chains

P10121 (FTSY_ECOLI) - Signal recognition particle receptor FtsY from Escherichia coli (strain K12)

Seq: Struc:					497 a.a. 283 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

		G-C-A-U-U-G-C-U-G-G-U-G-C-A-G-C-G-C-A-G-C-G-C-G-G-A-C-G-C-C-C-G-A-A-C-C-U-G-G- 102 bases
		G-C-A-U-U-G-C-U-G-G-U-G-C-A-G-C-G-C-A-G-C-G-C-G-G-A-C-G-C-C-C-G-A-A-C-C-U-G-G- 102 bases



		Enzyme reactions

Enzyme class:

Chains A, B, C, D: E.C.3.6.5.4 - signal-recognition-particle GTPase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

GTP + H2O = GDP + phosphate + H⁺

GTP

H2O

GDP
Bound ligand (Het Group name = GCP)
matches with 81.82% similarity

phosphate

H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

	Science 331:881-886 (2011)
PubMed id: 21330537

The crystal structure of the signal recognition particle in complex with its receptor.
S.F.Ataide, N.Schmitz, K.Shen, A.Ke, S.O.Shan, J.A.Doudna, N.Ban.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

23235881

K.Shen, S.Arslan, D.Akopian, T.Ha, and S.O.Shan (2012).
Activated GTPase movement on an RNA scaffold drives co-translational protein targeting.

Nature, 492, 271-275.

22056770

G.Bange, N.Kümmerer, P.Grudnik, R.Lindner, G.Petzold, D.Kressler, E.Hurt, K.Wild, and I.Sinning (2011).
Structural basis for the molecular evolution of SRP-GTPase activation by protein.

Nat Struct Mol Biol, 18, 1376-1380.

PDB code:

3syn

22086371

R.S.Hegde, and R.J.Keenan (2011).
Tail-anchored membrane protein insertion into the endoplasmic reticulum.

Nat Rev Mol Cell Biol, 12, 787-798.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.