PDBsum entry 2xrp

Structural protein

PDB id: 2xrp

Contents

Protein chains

426 a.a. *

429 a.a. *

95 a.a. *

Ligands

GDP ×4

GTP ×4

* Residue conservation analysis

PDB id:

2xrp

Name:

Structural protein

Title:

Human doublecortin n-dc repeat (1mjd) and mammalian tubulin (1jff and 3hke) docked into the 8-angstrom cryo-em map of doublecortin- stabilised microtubules

Structure:

Tubulin beta-2b chain. Chain: a, c, e, g. Synonym: tubulin beta chain. Tubulin alpha-1d chain. Chain: b, d, f, h. Synonym: tubulin alpha chain. Neuronal migration protein doublecortin. Chain: i. Fragment: residues 46-140.

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Organ: brain. Homo sapiens. Human. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Authors:

F.J.Fourniol,C.V.Sindelar,B.Amigues,D.K.Clare,G.Thomas,M.Perderiset, F.Francis,A.Houdusse,C.A.Moores

Key ref:

F.J.Fourniol et al. (2010). Template-free 13-protofilament microtubule-MAP assembly visualized at 8 A resolution. J Cell Biol, 191, 463-470. PubMed id: 20974813

Date:

18-Sep-10 Release date: 24-Nov-10

Protein chains

Q6B856  (TBB2B_BOVIN) -  Tubulin beta-2B chain from Bos taurus

Seq: 445 a.a.

Struc: 426 a.a.*

Protein chains

Q2HJ86  (TBA1D_BOVIN) -  Tubulin alpha-1D chain from Bos taurus

Seq: 452 a.a.

Struc: 429 a.a.*

Protein chain

O43602  (DCX_HUMAN) -  Neuronal migration protein doublecortin from Homo sapiens

Seq: 365 a.a.

Struc: 95 a.a.

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains A, C, E, G: E.C.3.6.5.6 - tubulin GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

GTP (Bound ligand (Het Group name = GTP) corresponds exactly) + H2O = GDP (Bound ligand (Het Group name = GDP) corresponds exactly) + phosphate + H(+)

• Enzyme class 2: Chains B, D, F, H: E.C.3.6.5.- - ?????
• Enzyme class 3: Chain I: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

J Cell Biol 191:463-470 (2010)

PubMed id: 20974813

Template-free 13-protofilament microtubule-MAP assembly visualized at 8 A resolution.

F.J.Fourniol, C.V.Sindelar, B.Amigues, D.K.Clare, G.Thomas, M.Perderiset, F.Francis, A.Houdusse, C.A.Moores.

ABSTRACT

No abstract given.