PDBsum entry 2xkv

Protein transport

PDB id: 2xkv

Contents

Protein chains

291 a.a. *

44 a.a. *

267 a.a. *

DNA/RNA

* Residue conservation analysis

PDB id:

2xkv

Name:

Protein transport

Title:

Atomic model of the srp-ftsy early conformation

Structure:

Signal recognition particle protein. Chain: a. Fragment: ng domain, residues 1-294. Synonym: fifty-four homolog. Engineered: yes. 4.5s RNA. Chain: b. Other_details: only the part of the 4.5s RNA that is visible in the em reconstruction is included.

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Authors:

L.F.Estrozi,D.Boehringer,S.-O.Shan,N.Ban,C.Schaffitzel

Key ref:

L.F.Estrozi et al. (2011). Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor. Nat Struct Biol, 18, 88-90. PubMed id: 21151118

Date:

13-Jul-10 Release date: 15-Dec-10

Protein chain

O07347  (SRP54_THEAQ) -  Signal recognition particle protein from Thermus aquaticus

Seq: 430 a.a.

Struc: 291 a.a.

Protein chain

P0AGD7  (SRP54_ECOLI) -  Signal recognition particle protein from Escherichia coli (strain K12)

Seq: 453 a.a.

Struc: 44 a.a.*

Protein chain

P83749  (FTSY_THEAQ) -  Signal recognition particle receptor FtsY from Thermus aquaticus

Seq: 304 a.a.

Struc: 267 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chain

C-U-C-U-G-U-U-G-G-U-U-C-U-C-U-G-U-G-C-U-C-U-G-U-G-C-U-C-U-G-U-U-U-A-C-C-A-G-G- 96 bases

Enzyme reactions

• Enzyme class: Chains A, C, D: E.C.3.6.5.4 - signal-recognition-particle GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Nat Struct Biol 18:88-90 (2011)

PubMed id: 21151118

Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.

L.F.Estrozi, D.Boehringer, S.O.Shan, N.Ban, C.Schaffitzel.

ABSTRACT

No abstract given.