UniProt functional annotation for Q10714

UniProt code: Q10714.

Organism: Drosophila melanogaster (Fruit fly).
Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
 
Function: May be involved in the specific maturation or degradation of a number of bioactive peptides. May play a role in the contractions of the heart, gut and testes, and in spermatid differentiation. {ECO:0000269|PubMed:12591244}.
 
Catalytic activity: Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II. {ECO:0000269|PubMed:7775412}.
Cofactor: Binds 1 zinc ion per subunit.
Enzyme regulation: Inhibited by captopril and, to a lesser extent, by lisinopril, trandolaprilat, fosinoprilat and enalaprilat. {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461}.
Biophysicochemical properties: Kinetic parameters: KM=33.5 uM for angiotensin I {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=53.4 uM for N-acetyl-Ser-Asp-Lys-Pro {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=2.59 mM for Hip-His-Leu {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=10.26 mM for Hip-His-Leu-NH(2) {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=372 uM for (Leu5)enkephalin {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949}; KM=1.88 mM for (Leu5)enkephalinamide {ECO:0000269|PubMed:7775412, ECO:0000269|PubMed:8761461, ECO:0000269|PubMed:9839949};
Subcellular location: Secreted, extracellular space.
Tissue specificity: Expressed in vesicular structures in spermatocytes and early spermatids (at protein level). {ECO:0000269|PubMed:12591244}.
Developmental stage: Expressed in the amnioserosa during germ band elongation, shortening and heart morphogenesis. Expressed in midgut throughout embryogenesis.
Ptm: Glycosylated. {ECO:0000269|PubMed:8761461}.
Disruption phenotype: Male flies are sterile. {ECO:0000269|PubMed:12591244}.
Similarity: Belongs to the peptidase M2 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.