PDBsum entry 2xbf

Ligase

PDB id

2xbf

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Contents

			Protein chain

					375 a.a.

			Ligands

					EDO ×4

			Metals

					_CA

			Waters ×197

PDB id:

2xbf

Links

Name:

Ligase

Title:

Nedd4 hect structure

Structure:

E3 ubiquitin-protein ligase nedd4. Chain: a. Fragment: hect domain, residues 519-900. Synonym: nedd-4, neural precursor cell expressed developmentally down-regulated protein 4, cell proliferation-inducing gene 53 protein. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: plyss.

Resolution:

2.50Å

R-factor:

0.200

R-free:

0.256

Authors:

E.Maspero,V.Cecatiello,A.Musacchio,S.Polo,S.Pasqualato

Key ref:

E.Maspero et al. (2011). Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation. Embo Rep, 12, 342-349. PubMed id: 21399620

Date:

09-Apr-10

Release date:

23-Mar-11

PROCHECK

	Headers

	References

Protein chain

P46934 (NEDD4_HUMAN) - E3 ubiquitin-protein ligase NEDD4 from Homo sapiens

Seq: Struc:

Seq: Struc:

Seq: Struc:					1319 a.a. 375 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

	Embo Rep 12:342-349 (2011)
PubMed id: 21399620

Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation.
E.Maspero, S.Mari, E.Valentini, A.Musacchio, A.Fish, S.Pasqualato, S.Polo.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22820888

Y.Kulathu, and D.Komander (2012).
Atypical ubiquitylation - the unexplored world of polyubiquitin beyond Lys48 and Lys63 linkages.

Nat Rev Mol Cell Biol, 13, 508-523.

21947082

M.Inui, A.Manfrin, A.Mamidi, G.Martello, L.Morsut, S.Soligo, E.Enzo, S.Moro, S.Polo, S.Dupont, M.Cordenonsi, and S.Piccolo (2011).
USP15 is a deubiquitylating enzyme for receptor-activated SMADs.

Nat Cell Biol, 13, 1368-1375.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.