PDBsum entry 2xbb

Ligase/protein binding

PDB id

2xbb

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Contents

			Protein chains

					376 a.a.


					76 a.a.

			Ligands

					GOL ×2

			Waters ×135

PDB id:

2xbb

Links

Name:

Ligase/protein binding

Title:

Nedd4 hect:ub complex

Structure:

E3 ubiquitin-protein ligase nedd4. Chain: a, b. Fragment: hect domain, residues 519-900. Synonym: nedd-4, neural precursor cell expressed developmentally down-regulated protein 4, cell proliferation-inducing gene 53 protein. Engineered: yes. Ubiquitin. Chain: c, d

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: plyss. Bos taurus. Cattle. Organism_taxid: 9913.

Resolution:

2.68Å

R-factor:

0.207

R-free:

0.249

Authors:

E.Maspero,V.Cecatiello,A.Musacchio,S.Polo,S.Pasqualato

Key ref:

E.Maspero et al. (2011). Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation. Embo Rep, 12, 342-349. PubMed id: 21399620

Date:

08-Apr-10

Release date:

23-Mar-11

PROCHECK

	Headers

	References

Protein chains

P46934 (NEDD4_HUMAN) - E3 ubiquitin-protein ligase NEDD4 from Homo sapiens

Seq: Struc:

Seq: Struc:

Seq: Struc:					1319 a.a. 376 a.a.

Protein chains

P63048 (RL40_BOVIN) - Ubiquitin-ribosomal protein eL40 fusion protein from Bos taurus

Seq: Struc:					128 a.a. 76 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

Chains A, B: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

	Embo Rep 12:342-349 (2011)
PubMed id: 21399620

Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation.
E.Maspero, S.Mari, E.Valentini, A.Musacchio, A.Fish, S.Pasqualato, S.Polo.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

22820888

Y.Kulathu, and D.Komander (2012).
Atypical ubiquitylation - the unexplored world of polyubiquitin beyond Lys48 and Lys63 linkages.

Nat Rev Mol Cell Biol, 13, 508-523.

21947082

M.Inui, A.Manfrin, A.Mamidi, G.Martello, L.Morsut, S.Soligo, E.Enzo, S.Moro, S.Polo, S.Dupont, M.Cordenonsi, and S.Piccolo (2011).
USP15 is a deubiquitylating enzyme for receptor-activated SMADs.

Nat Cell Biol, 13, 1368-1375.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.