PDBsum entry 2xb1

Transcription

PDB id: 2xb1

Contents

Protein chains

96 a.a. *

Ligands

GOL ×2

PEG

Metals

_ZN ×4

Waters ×189

* Residue conservation analysis

PDB id:

2xb1

Name:

Transcription

Title:

Crystal structure of the human pygo2 phd finger in complex with the b9l hd1 domain

Structure:

Pygopus homolog 2, b-cell cll/lymphoma 9-like protein. Chain: a, c. Fragment: phd finger and hd1 domain, residues 325-387,232-266. Synonym: pygopus 2, b-cell lymphoma 9-like protein, bcl9-like protein, bcl9-2, b9l. Engineered: yes. Other_details: artificial linker between phd finger and hd1 domain (7aa linker-gsgsgsg)

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: codonplus-ril.

Resolution:

1.90Å R-factor: 0.174 R-free: 0.217

Authors:

T.C.Miller,T.J.Rutherford,C.M.Johnson,M.Fiedler,M.Bienz

Key ref:

T.C.Miller et al. (2010). Allosteric remodelling of the histone H3 binding pocket in the Pygo2 PHD finger triggered by its binding to the B9L/BCL9 co-factor. J Mol Biol, 401, 969-984. PubMed id: 20637214

Date:

01-Apr-10 Release date: 28-Jul-10

Protein chains

Q86UU0  (BCL9L_HUMAN) -  B-cell CLL/lymphoma 9-like protein from Homo sapiens

Seq: 1499 a.a.

Struc: 96 a.a.*

Protein chains

Q9BRQ0  (PYGO2_HUMAN) -  Pygopus homolog 2 from Homo sapiens

Seq: 406 a.a.

Struc: 96 a.a.*

* PDB and UniProt seqs differ at 75 residue positions (black crosses)

J Mol Biol 401:969-984 (2010)

PubMed id: 20637214

Allosteric remodelling of the histone H3 binding pocket in the Pygo2 PHD finger triggered by its binding to the B9L/BCL9 co-factor.

T.C.Miller, T.J.Rutherford, C.M.Johnson, M.Fiedler, M.Bienz.

ABSTRACT

No abstract given.