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PDBsum entry 2x25

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Isomerase PDB id
2x25
Jmol
Contents
Protein chain
168 a.a.
Waters ×443

References listed in PDB file
Key reference
Title Acetylation regulates cyclophilin a catalysis, Immunosuppression and HIV isomerization.
Authors M.Lammers, H.Neumann, J.W.Chin, L.C.James.
Ref. Nat Chem Biol, 2010, 6, 331-337.
PubMed id 20364129
Abstract
Cyclophilin A (CypA) is a ubiquitous cis-trans prolyl isomerase with key roles in immunity and viral infection. CypA suppresses T-cell activation through cyclosporine complexation and is required for effective HIV-1 replication in host cells. We show that CypA is acetylated in diverse human cell lines and use a synthetically evolved acetyllysyl-tRNA synthetase/tRNA(CUA) pair to produce recombinant acetylated CypA in Escherichia coli. We determined atomic-resolution structures of acetylated CypA and its complexes with cyclosporine and HIV-1 capsid. Acetylation markedly inhibited CypA catalysis of cis to trans isomerization and stabilized cis rather than trans forms of the HIV-1 capsid. Furthermore, CypA acetylation antagonized the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition. Our results reveal that acetylation regulates key functions of CypA in immunity and viral infection and provide a general set of mechanisms by which acetylation modulates interactions to regulate cell function.
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