PDBsum entry 2wma

Cell cycle

PDB id: 2wma

Contents

Protein chains

297 a.a. *

258 a.a. *

266 a.a. *

Ligands

ARG-LYS-LEU-PHE-ASN

Waters ×95

* Residue conservation analysis

PDB id:

2wma

Name:

Cell cycle

Title:

Structural and thermodynamic consequences of cyclization of peptide ligands for the recruitment site of cyclin a

Structure:

Cell division protein kinase 2. Chain: a, c. Synonym: cdk2, p33 protein kinase. Engineered: yes. Cyclin-a2. Chain: b, d. Fragment: residues 174-432. Synonym: cyclin-a. Engineered: yes.

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 511693. Synthetic: yes. Organism_taxid: 9606

Resolution:

2.80Å R-factor: 0.215 R-free: 0.285

Authors:

G.F.Robertson,J.A.Endicott,M.E.M.Noble,J.M.Mcdonnell

Key ref:

G.F.Robertson et al. Structural and thermodynamic consequences of cyclizat peptide ligands for the recruitment site of cyclin a. To be published, .

Date:

30-Jun-09 Release date: 01-Sep-10

Protein chain

P24941  (CDK2_HUMAN) -  Cyclin-dependent kinase 2 from Homo sapiens

Seq: 298 a.a.

Struc: 297 a.a.*

Protein chains

P20248  (CCNA2_HUMAN) -  Cyclin-A2 from Homo sapiens

Seq: 432 a.a.

Struc: 258 a.a.

Protein chain

P24941  (CDK2_HUMAN) -  Cyclin-dependent kinase 2 from Homo sapiens

Seq: 298 a.a.

Struc: 266 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, C: E.C.2.7.11.22 - cyclin-dependent kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site